Extraction and analysis of bioactive compound from freshwater crab tissue

The adult species of freshwater crabs (Berytephusa Cuniclaris) were collected from the Northern region Maharashtra, India, and isolated, refrigerated in laboratory. living euthanized at -30°C by thermal shock for 15 minutes. then clearly dissented tissue was extracted crab’s abdomen, crude extract obtained their tissue. completely dried off microwave pulverized a cold maceration process used immersing powder into ethyl alcohol maintaining stability bioactive compounds low temperature vacuum-packed 21 days room temperature. Stirring given infrequently due to raising up speed extraction compound diffuses cell anticipation equilibrium reached. repetitive filtration done refine concentrated with rotator evaporators eliminate excess amount ethanol which form pure product. current study is described first time India help an advanced analytical tool like UV, FTIR, NMR, LCMS, Single-crystal analysis, antimicrobial potencies that crab beneficial human as well medicinal source presence cortisol, aldosterone, isoleucine, estradiol most isolated dexamethasone, testosterone, norethisterone have existed cluster form.